Bioconjugate Techniques by Greg T. Hermanson PDF

By Greg T. Hermanson

ISBN-10: 012342335X

ISBN-13: 9780123423351

ISBN-10: 0123886236

ISBN-13: 9780123886231

Bioconjugate Techniques is the basic consultant to the amendment and crosslinking of biomolecules to be used in study, diagnostics, and therapeutics. It presents hugely precise details at the chemistry, reagent platforms, and useful functions for growing classified or conjugate molecules. It additionally describes dozens of reactions with information on countless numbers of commercially to be had reagents and using those reagents for enhancing or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and artificial polymers. Armed with this knowledge and the plentiful protocols supplied, readers will shape certain complexes that may be used for detecting, quantifying, and focusing on vital analytes.
This publication is helping readers make: excessive job antibody-enzymes conjugates, immunotoxins, immunogen complexes, liposome conjugates; in addition to biotinylated molecules, avidin or streptavidin conjugates, colloidal gold classified proteins, PEG or dextran complexes, categorized oligonucleotide probes, and fluorescently tagged or radiolabeled molecules.

  • This publication is the 1st to entirely trap the total box of bioconjugate chemistry in one volume
  • Serves as a realistic consultant to amendment and cross-linking know-how for study, diagnostics, and therapeutics
  • Provides priceless, targeted, easy-to-follow, step by step protocols
  • Contains easy-to-read, and easy-to-understand key innovations for making bioconjugates of all types
  • Efficiently covers the chemistry of bioconjugation, the most important reagents to be had for amendment and cross-linking, and the appliance of those reagents to the synthesis of hugely energetic conjugates
  • Cites over greater than references keyed to ideas coated within the book
  • Uses greater than six hundred figures to demonstrate bioconjugate reagents, their reactions, and applications
  • Suggests assets for all key reagents

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Bioconjugate Techniques by Greg T. Hermanson

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